01480nam a2200205Ia 4500001077400000002005100774008003700825010001100862530002200873583002600895650003500921650004200956650004100998650003001039650004401069650005501113700003301168700003801201700003501239IA_Isolation of Rhizobium, was made from nodules of alfalfa. In the laboratory, washed and disinfected the nodules and then crushed in a solution of 3% peptone. Of this solution with rhizobia, were taken handle bacterial and planted in the middle ELMA. The plates were incubated at 35 ° C for 48 hours. Then made periodic replanting in the same culture medium for purification of the strain. Was determined the colonial and microscopic morphology of the strain, and subsequently cultured in liquid (broth yeast extract-mannitol), the incubation time was 72 hours, at the end of which, cells were separated by centrifugation at 1500 rpm for 15 minutes, performing three washes with NaCl21%.The cell pack was refrigerated for subsequent development and testing of inoculant.ELABORACION DE UN INOCULANTE PARA ALFALFA (MEDICAG220117s    xx            000 0 und d  a184353  aDisponible en PDF  a1 ej. imp. ; 2 ej. CD  aAlfalfaxInoculation methods    aAlfalfaxMétodos de inoculación    aBacterias fijadoras de nitrógeno    aNitrogenxfixing bacteria  aPlant growthxpromoting rhizobacteria   0aRizobacterias promotoras del crecimiento vegetal    aLeyva Ruelas, Gabriel, dir.   aPonce Hernández, Pedro, asesor   aReyna Izaguirre, Diana, asesor