01614nam a2200229Ia 4500001077400000002005100774008003700825010001100862530002200873583002600895650004300921650005000964650004901014650003801063650005201101650006301153700004101216700004601257700004301303942001901346999001901365IA_Isolation of Rhizobium, was made from nodules of alfalfa. In the laboratory, washed and disinfected the nodules and then crushed in a solution of 3% peptone. Of this solution with rhizobia, were taken handle bacterial and planted in the middle ELMA. The plates were incubated at 35 ° C for 48 hours. Then made periodic replanting in the same culture medium for purification of the strain. Was determined the colonial and microscopic morphology of the strain, and subsequently cultured in liquid (broth yeast extract-mannitol), the incubation time was 72 hours, at the end of which, cells were separated by centrifugation at 1500 rpm for 15 minutes, performing three washes with NaCl21%.The cell pack was refrigerated for subsequent development and testing of inoculant.ELABORACION DE UN INOCULANTE PARA ALFALFA (MEDICAG220117s    xx            000 0 und d  a184353  aDisponible en PDF  a1 ej. imp. ; 2 ej. CD  aAlfalfaxInoculation methods  9156763  aAlfalfaxMétodos de inoculación  9156764  aBacterias fijadoras de nitrógeno  9156765  aNitrogenxfixing bacteria9156766  aPlant growthxpromoting rhizobacteria  9156767 0aRizobacterias promotoras del crecimiento vegetal  9156768  aLeyva Ruelas, Gabriel, dir. 9148473  aPonce Hernández, Pedro, asesor 9163694  aReyna Izaguirre, Diana, asesor9163710  cTESIS L86 2011  c184353d184353