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  <titleInfo>
    <title>Caracterización metagenómica y peptidómica de leche fermentada con granos de kefir</title>
  </titleInfo>
  <titleInfo type="uniform">
    <title>Metagenomic and peptidomic characterization of milk fermented whith kefir grains.  Español</title>
  </titleInfo>
  <name type="personal">
    <namePart>Ortiz Trapala, Karla</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Lobato Calleros, Consuelo Silvia Olivia</namePart>
    <role>
      <roleTerm type="text">directora</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Hernández Rodríguez, Landy</namePart>
    <role>
      <roleTerm type="text">asesora</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Aguirre Mandujano, Eleazar</namePart>
    <role>
      <roleTerm type="text">asesor</roleTerm>
    </role>
  </name>
  <typeOfResource>text</typeOfResource>
  <genre authority="marc">theses</genre>
  <originInfo>
    <place>
      <placeTerm type="code" authority="marccountry">mx</placeTerm>
    </place>
    <dateIssued encoding="marc">2023</dateIssued>
    <issuance>monographic</issuance>
  </originInfo>
  <language>
    <languageTerm authority="iso639-2b" type="code">spa</languageTerm>
  </language>
  <language objectPart="summary or subtitle">
    <languageTerm authority="iso639-2b" type="code">eng</languageTerm>
  </language>
  <physicalDescription>
    <extent>1 recurso en línea (xiv, 85 páginas) : ilustraciones, tablas, gráficas, fotografías</extent>
  </physicalDescription>
  <abstract>Las leches fermentadas (LFs) forman parte del grupo de alimentos funcionales  ya que contienen microorganismos con potencial probiótico. El kéfir es una  antigua bebida láctea a la cual se le atribuyen beneficios al ser consumido, v.g la  reducción de síntomas asociados a la intolerancia de la lactosa y estimulación  del sistema inmunológico. Se caracterizaron fisicoquímicamente, metagenómica  y peptidómicamente cuatro leches fermentadas (LFA, LFB, LFC y LFD) con  granos de kéfir. Los parámetros de acidez variaron de 3.76 a 3.82 %. Después  de la fermentación (48 h), las BAL estuvieron presentes de 7.25 y 11.13 log10  UFC · mL-1 y las levaduras de 6.05 y 7.93 log10 UFC · mL-1. Así mismo, se  estudió la microbiota de LFA y LFD mediante la técnica de secuenciación  metagenómica shotgun, la cual reveló 77 géneros de microorganismos, donde  los más representativos fueron Acetobacter (73.8 %), Lactobacillus (19.6 %),  Lentilactobacillus (3.9 %), Lactococcus (0.76 %), de igual manera las especies  con mayor abundancia fueron Lactobacillus kefiranofaciens, Acetobacter  orientalis, Lactobacillus helvéticus, Staphylococcus aureus, Acetobacter syzygii,  Acetobacter okinawensis, Lentilactobacillus kefiri y Lactococcus lactis. El perfil  peptídico utilizando la técnica de cromatografía líquida acoplada a espectrometría  de masas (LC-MS/MS) mostró la presencia de 3691 péptidos. Las principales  actividades biológicas, determinadas in silico, fueron antinflamatoria, inhibidora  de ACE, antibacterial, inhibidor de DPP – III y DPP – IV, antitrombótico, anticancerígeno y antioxidante. Igualmente se evaluó la actividad antioxidante in  vitro con los ensayos ABTS y DPPH; LFB demostró mayor porcentaje de  actividad inhibidora de radicales libres (55.45 %). Los resultados obtenidos  demuestran que, a las 48 de fermentación, los microorganismos presentes en la  leche fermentadas liberan péptidos con actividad biológica. --</abstract>
  <abstract>Fermented milks (LFs) are part of the group of functional foods since they contain  microorganisms with probiotic potential. Kefir is an ancient dairy drink to which benefits  are attributed when consumed, e.g. the reduction of symptoms associated with lactose  ntolerance and stimulation of the immune system. Four fermented milks (LFA, LFB, LFC  and LFD) with kefir grains were characterized physiochemically, metagenomically and  peptidomically. The acidity parameters varied from 3.76 to 3.82%. After fermentation (48  h), the BAL were present of 7.25 and 11.13 log10 CFU ·mL-1 and the yeasts of 6.05 and  7.93 log10 CFU · mL-1. Likewise, the microbiota of LFA and LFD was studied using the  shotgun metagenomic sequencing technique, which revealed 77 genera of  microorganisms, where the most representative were Acetobacter (73.8%), Lactobacillus  (19.6%), Lentilactobacillus (3.9%), Lactococcus (0.76%), likewise the species with the  highest abundance were Lactobacillus kefiranofaciens, Acetobacter orientalis,  Lactobacillus helveticus, Staphylococcus aureus, Acetobacter syzygii, Acetobacter  okinawensis, Lentilactobacillus kefiri and Lactococcus lactis. The peptide profile by the  liquid chromatography coupled to mass spectrometry (LC-MS/MS) technique showed the  presence of 3691 peptides. The main biological activities, determined in silico, were antiinflammatory, ACE inhibitor, antibacterial, DPP–III and DPP–IV inhibitor, antithrombotic,  anticancer and antioxidant). The antioxidant activity was also evaluated in vitro with the ABTS and DPPH tests; LFB showed a higher percentage of free radical inhibitory activity  (55.45%). The results obtained show that, at 48 of fermentation, the microorganisms  present in fermented milk release peptides with biological activity.</abstract>
  <targetAudience authority="marctarget">specialized</targetAudience>
  <note type="statement of responsibility">Karla Ortiz Trapala</note>
  <note>Tesis MCTA Agroindustrias 2023 MAE</note>
  <note>Incluye bibliográfías</note>
  <subject authority="nal">
    <topic>Leche fermentada</topic>
    <topic>Evaluación sensorial de los alimentos</topic>
  </subject>
  <subject authority="nal">
    <topic>Kefir</topic>
    <topic>Evaluación sensorial de los alimentos</topic>
  </subject>
  <subject authority="nal">
    <topic>Fermented milk</topic>
    <topic>Sensory evaluation</topic>
  </subject>
  <subject authority="nal">
    <topic>Kefir</topic>
    <topic>Sensory evaluation</topic>
  </subject>
  <identifier type="uri">http://10.13.5.2/tesis/tm/ORTIZ_TRAPALA_KARLA.pdf</identifier>
  <location>
    <url displayLabel="DESCARGAR PDF">http://10.13.5.2/tesis/tm/ORTIZ_TRAPALA_KARLA.pdf</url>
  </location>
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    <recordContentSource authority="marcorg"/>
    <recordCreationDate encoding="marc">250112</recordCreationDate>
    <recordChangeDate encoding="iso8601">20250707172804.0</recordChangeDate>
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