000			01651nam a2200241Ia 4500
001			IA_Isolation of Rhizobium, was made from nodules of alfalfa. In the laboratory, washed and disinfected the nodules and then crushed in a solution of 3% peptone. Of this solution with rhizobia, were taken handle bacterial and planted in the middle ELMA. The plates were incubated at 35 ° C for 48 hours. Then made periodic replanting in the same culture medium for purification of the strain. Was determined the colonial and microscopic morphology of the strain, and subsequently cultured in liquid (broth yeast extract-mannitol), the incubation time was 72 hours, at the end of which, cells were separated by centrifugation at 1500 rpm for 15 minutes, performing three washes with NaCl21%.The cell pack was refrigerated for subsequent development and testing of inoculant.
002			ELABORACION DE UN INOCULANTE PARA ALFALFA (MEDICAG
008			220117s xx 000 0 und d
010			_a184353
530			_aDisponible en PDF
583			_a1 ej. imp. ; 2 ej. CD
650			_aAlfalfa _xInoculation methods _9156763
650			_aAlfalfa _xMétodos de inoculación _9156764
650			_aBacterias fijadoras de nitrógeno _9156765
650			_aNitrogen _xfixing bacteria _9156766
650			_aPlant growth _xpromoting rhizobacteria _9156767
650		0	_aRizobacterias promotoras del crecimiento vegetal _9156768
700			_aLeyva Ruelas, Gabriel, dir. _9148473
700			_aPonce Hernández, Pedro, asesor _9163694
700			_aReyna Izaguirre, Diana, asesor _9163710
942			_cTESIS L86 2011
999			_c184353 _d184353