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Efecto antibacteriano de aislamientos de bacterias ácido lácticas sobre Helicobacter pylori in vitro / Martha María Juárez Rodríguez

By: Contributor(s): Material type: TextLanguage: Spanish Summary language: English Chapingo, México : El autor, 2016Description: iv, 68 hojas : ilustraciones, gráficas, diagramas, tablas, fotos + 1 CD-ROMContent type:
  • texto
Media type:
  • no mediado
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  • volumen
Uniform titles:
  • In vitro antibacterial effect of isolated lactic acid bacteria on Helicobacter pylori. Español
Subject(s): Online resources: Dissertation note: Tesis (Ingeniero Agroindustrial) -- UACh. Departamento de Ingeniería Agroindustrial, 2016 Abstract: Helicobacter pylori es considerado un patógeno altamente prevalente relacionado con enfermedades gastrointestinales, sobre todo en países emergentes, con porcentajes de infección mayores al 75 %. Debido a que cada vez más, dicho microorganismo genera resistencia a distintos tratamientos realizados con antibióticos, surge como alternativa para su erradicación el uso de bacterias probióticas como lo son las bacterias ácido lácticas, pues existe evidencia de que cepas del género Lactobacillus y Bifidobacterium tienen la capacidad de inhibir a la bacteria de estudio. En la presente investigación, a partir de distintos productos alimenticios, se logró aislar 24 cepas de bacterias ácido lácticas a través de la evaluación de crecimiento macroscópico, actividad oxidasa y catalasa, tinción de Gram, movilidad y crecimiento en condiciones anaerobias. Sólo cuatro fueron seleccionadas, después de las pruebas de resistencia en medios hostiles como pH 3 y 0.30 % de sales biliares. Dichos aislamientos de bacterias ácido lácticas mostraron actividad antimicrobiana sobre Helicobacter pylori cuando se redujo su proceso fermentativo desarrollándolos en un medio con baja concentración de dextrosa, siendo el mejor tratamiento un cultivo multiespecie de aislamientos derivados de col fermentada y kéfir --Abstract: Helicobacter pylori is considered to be among the major pathogenic bacterium responsible for gastrointestinal chronic diseases in humans. It has infected around 75% of the population in emerging countries. In recent years, H. pylori has gradually developed antibiotic resistance making it more challenging for physicians to treat diseases caused by this particular bacterium as it can generate undesired side effects of clinical therapy as well as the treatment is difficult to afford. For these reasons, there is an urgent need to research, create and develop different alternative solutions to prevent and treat diseases from H. pylori. Recently, different studies have demonstrated that the use and ingest of probiotics (lactic acid producing bacteria) such as Lactobacillus and Bifidobacterium have the ability to inhibit H. pylori. In this study, different types of edible products (raw milk, kéfir, sauerkraut and two commercial fermented milk) were used as source for isolating lactic acid bacteria. From this, a total of 24 lactic acid strains were isolated taking into account their catalase and oxidase activity, Gram stain and anaerobically. Nonetheless, only four lactic acid bacteria strains were carefully selected after being tested for resistance and tolerance to acidifying activity, low pH values and bile salts for instance acidic pH of 3 and low bile salt concentration (0.30 %). The results demonstrated that the lactic acid bacteria strain isolated from the mix of cabbage and fermented milk (kéfir) showed the highest antibacterial activity, thus, inhibiting H. pylori
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Cover image Item type Current library Home library Collection Shelving location Call number Materials specified Vol info URL Copy number Status Notes Date due Barcode Item holds Item hold queue priority Course reserves
Tesis digital Ingenieria Agroindustrial En línea Tesis digitales de Licenciatura Available 1201010374
CD Ingenieria Agroindustrial Tesis Tesis J83 2016 (Browse shelf(Opens below)) Ej. 2 Available (Préstamo interno) 1101010375
Tesis Ingenieria Agroindustrial Tesis Tesis J83 2016 (Browse shelf(Opens below)) Ej. 1 Available (Préstamo interno) 1101010374

El CD contiene reporte y seminario

Tesis (Ingeniero Agroindustrial) -- UACh. Departamento de Ingeniería Agroindustrial, 2016

Bibliografía: hojas 58-66

Helicobacter pylori es considerado un patógeno altamente prevalente relacionado con enfermedades gastrointestinales, sobre todo en países emergentes, con porcentajes de infección mayores al 75 %. Debido a que cada vez más, dicho microorganismo genera resistencia a distintos tratamientos realizados con antibióticos, surge como alternativa para su erradicación el uso de bacterias probióticas como lo son las bacterias ácido lácticas, pues existe evidencia de que cepas del género Lactobacillus y Bifidobacterium tienen la capacidad de inhibir a la bacteria de estudio. En la presente investigación, a partir de distintos productos alimenticios, se logró aislar 24 cepas de bacterias ácido lácticas a través de la evaluación de crecimiento macroscópico, actividad oxidasa y catalasa, tinción de Gram, movilidad y crecimiento en condiciones anaerobias. Sólo cuatro fueron seleccionadas, después de las pruebas de resistencia en medios hostiles como pH 3 y 0.30 % de sales biliares. Dichos aislamientos de bacterias ácido lácticas mostraron actividad antimicrobiana sobre Helicobacter pylori cuando se redujo su proceso fermentativo desarrollándolos en un medio con baja concentración de dextrosa, siendo el mejor tratamiento un cultivo multiespecie de aislamientos derivados de col fermentada y kéfir --

Helicobacter pylori is considered to be among the major pathogenic bacterium responsible for gastrointestinal chronic diseases in humans. It has infected around 75% of the population in emerging countries. In recent years, H. pylori has gradually developed antibiotic resistance making it more challenging for physicians to treat diseases caused by this particular bacterium as it can generate undesired side effects of clinical therapy as well as the treatment is difficult to afford. For these reasons, there is an urgent need to research, create and develop different alternative solutions to prevent and treat diseases from H. pylori. Recently, different studies have demonstrated that the use and ingest of probiotics (lactic acid producing bacteria) such as Lactobacillus and Bifidobacterium have the ability to inhibit H. pylori. In this study, different types of edible products (raw milk, kéfir, sauerkraut and two commercial fermented milk) were used as source for isolating lactic acid bacteria. From this, a total of 24 lactic acid strains were isolated taking into account their catalase and oxidase activity, Gram stain and anaerobically. Nonetheless, only four lactic acid bacteria strains were carefully selected after being tested for resistance and tolerance to acidifying activity, low pH values and bile salts for instance acidic pH of 3 and low bile salt concentration (0.30 %). The results demonstrated that the lactic acid bacteria strain isolated from the mix of cabbage and fermented milk (kéfir) showed the highest antibacterial activity, thus, inhibiting H. pylori

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